Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TLR6 in samples. An antibody specific for TLR6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTLR6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLR6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLR6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Toll Like Receptor 6 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. By screening a human placenta library with a mouse Tlr6 probe, Takeuchi et al. (1999) isolated a cDNA encoding human TLR6. The deduced 796-amino acid type I transmembrane protein, which is 74% identical to mouse Tlr6 and 69% identical to TLR1, contains an N-terminal signal peptide, 20 tandemly repeated extracellular leucine-rich motifs, and a cytoplasmic domain homologous to IL1R, as seen in other TLRs. RT-PCR analysis detected Tlr6 in mouse thymus, spleen, ovary, and lung.
