Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC25A37 in samples. An antibody specific for SLC25A37 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC25A37 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC25A37 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC25A37 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:SLC25A37 belongs to the SLC25 family of mitochondrial carrier proteins.Shaw et al. (2006) cloned mouse Slc25a37, which they called mitoferrin. Northern blot analysis detected expression in all tissues examined, with highest levels in hematopoietic organs, fetal liver, bone marrow, and spleen. In developing mouse embryos, mitoferrin was first detected at embryonic day 7.5 in the extraembryonic yolk sac, coincident with the appearance of blood islands.
By searching databases for SLC25 family members, Haitina et al. (2006) identified 2 splice variants of SLC25A37. The deduced protein contains 351 amino acids.SLC25A37 gene maps to chromosome 8p21. The mouse Slc25a37 gene maps to chromosome 14.
