Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SEMA5A in samples. An antibody specific for SEMA5A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA5A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA5A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA5A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:SEMA5A belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.
The deduced 1,077-amino acid protein contains an N-terminal signal sequence, followed by a semaphorin domain, 7 thrombospondin type-1 repeats, a putative transmembrane domain, and a short cytoplasmic tail. It also has 13 conserved cysteines and a conserved N-glycosylation site. Northern blot analysis of whole mouse embryos detected 2 Semf transcripts at embryonic day 10, and their expression decreased toward birth.
