Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SCYL1 in samples. An antibody specific for SCYL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCYL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCYL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCYL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Chemokine (C-C motif) ligand 13 (CCL13) is a small cytokine belonging to the CC chemokine family.The chemokines are secreted proteins involved in immunoregulatory and inflammatory processes. Naruse et al. (1996) identified on chromosome 17q11.2 a cluster of chemokines from the CC family (characterized by a cys-cys sequence motif) and distinguished from a second chemokine family that features a cys-xxx-cys (CXC) motif . They mapped 1 gene of the CC family (called NCC1 by them) to the cluster using a panel of somatic cell hybrids with known deletions. NCC1 was originally characterized as an expressed sequence tag (EST). The deduced order of genes from a YAC contig assembled for the region is cen--NF1--MCP3, MCP1, NCC1, I-309--Y1741 breakpoint--RANTES--LD78-gamma, AT744.2 , LD78-beta --NCC3, NCC2, AT744.1 ), LD78-alpha--NCC4--RARA --tel.
