Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SCLY in samples. An antibody specific for SCLY has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCLY present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCLY is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCLY bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Selenocysteine lyase (SCLY; EC 4.4.1.16) catalyzes the pyridoxal 5-prime phosphate-dependent conversion of L-selenocysteine to L-alanine and elemental selenium.
The deduced mouse protein has 432 amino acids. RT-PCR detected Scly expression in mouse brain, heart, lung, stomach, liver, kidney, spleen, and testis. Western blot analysis detected expression in all mouse tissues examined, with the highest expression in liver, kidney, and testis. Mouse Scly localized to the cytosolic fraction in mouse liver and formed homodimers.Scly catalyzed the conversion of L-selenocysteine to L-alanine. Scly activity required pyridoxal 5-prime phosphate, was specific to L-selenocysteine, and showed maximum reactivity at pH 9.0.
