Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SACM1L in samples. An antibody specific for SACM1L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySACM1L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SACM1L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SACM1L bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:SAC1, a 587-amino acid protein with 34% sequence identity with the yeast RSD1 (recessive suppressor of secretory defect) protein. RT-PCR detected expression of SAC1 in heart, brain, lung, liver, kidney, pancreas, testis, and ovary, and less abundant expression in skeletal muscle and spleen. mouse Sac1 which encodes a protein sharing 95% sequence identity with human SAC1. Both contain 2 transmembrane regions and a leucine zipper pattern. Both are predicted to be cytoplasmic proteins in the endoplasmic reticulum. Northern blot analysis in mouse showed high expression of a 4-kb transcript in brain, lung, liver, and kidney. A lower level of expression was seen in spleen and skeletal muscle, and no expression was observed in heart or testis.
