Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate RXRG in samples. An antibody specific for RXRG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRXRG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RXRG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RXRG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:RXR-gamma), also known as NR2B3 (nuclear receptor subfamily 2, group B, member 3) is a nuclear receptor encoded by the RXRG gene.
This gene encodes a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the antiproliferative effects of retinoic acid (RA). This receptor forms homodimers with the retinoic acid, thyroid hormone, and vitamin D receptors, increasing both DNA binding and transcriptional function on their respective response elements. This gene is expressed at significantly lower levels in non-small cell lung cancer cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
