Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate RELL1 in samples. An antibody specific for RELL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRELL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RELL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RELL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The predicted RELL1 sequence contains a transmembrane domain but lacks extracellular cysteine-rich domains. RELL1 shares 32% and 40% amino acid identity with RELT and RELL2, respectively. Northern blot analysis detected an approximately 4-kb transcript in all human tissues examined with highest expression in placenta, spleen, skeletal muscle, and testis. Immunofluorescent confocal microscopy localized RELL1 to the plasma membrane in COS-7 cells and colocalized RELL1 with RELL2 and RELT.
Coimmunoprecipitation studies showed that RELL1 interacted with RELL2, RELT, and OSR1. By in vitro kinase assay, Cusick et al. (2006) showed that RELL1, RELL2, and RELT were phosphorylated by OSR1.
