Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate RAB8A in samples. An antibody specific for RAB8A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAB8A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAB8A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAB8A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:RAB8A is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane.
This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins.
