Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PTX3 in samples. An antibody specific for PTX3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTX3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTX3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTX3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:PTX3 bound selected pathogens, including Aspergillus fumigatus, Pseudomonas aeruginosa, and Salmonella typhimurium. PTX3 bound to either viable or heat-inactivated conidia, but not the hyphae, of A. fumigatus, and Ptx3 facilitated conidia interaction with mononuclear phagocytes. Conidia rapidly induced PTX3 production in human and mouse monocytes and dendritic cells, but not in neutrophils, fibroblasts, or endothelial or epithelial cells. Infection of mice and humans with A. fumigatus induced high levels of PTX3 in plasma and bronchoalveolar lavage fluid. Significant levels of PTX3 were detected in plasma of neutropenic patients with systemic A. fumigatus infection, but only low levels were found in control subjects.
