Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PRSS27 in samples. An antibody specific for PRSS27 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRSS27 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSS27 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS27 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Pancreasin is a pancreatic tryptic serine peptidase that cleaves peptides after an arginine residue. The deduced 290-amino acid preproprotease contains an N-terminal signal peptide, followed by a prosequence and a 256-amino acid serine protease catalytic domain. The catalytic domain contains the essential catalytic triad (his75, asp124, and ser229) conserved in serine proteases, as well as 2 putative N-glycosylation sites. Northern blot analysis revealed expression of pancreasin only in pancreas. RT-PCR detected expression in several other tissues, including strong expression in lung and placenta. Immunocytochemical analysis of pancreatic carcinoma cells revealed cytoplasmic reactivity, and transfected Chinese hamster ovary cells secreted pancreasin into the medium.
