Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PRPF19 in samples. An antibody specific for PRPF19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRPF19 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRPF19 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRPF19 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:PSO4 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair . PSO4 shares 38% amino acid identity with S. cerevisiae Pso4. SDS-PAGE indicated that PSO4 has an apparent molecular mass of 55 kD.PSO4 readily bound plasmid DNA substrates, including closed circular plasmid DNA, indicating that PSO4 binds in a sequence-independent manner and does not require free DNA ends for binding. PSO4 expression was induced 15- to 30-fold in a normal human fibroblast cell line by gamma irradiation and chemical mutagens, but not by ultraviolet irradiation. Loss of PSO4 expression induced by small interfering RNA resulted in accumulation of double-strand breaks, apoptosis, and decreased cell survival after DNA damage.
