Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PRKRIR in samples. An antibody specific for PRKRIR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKRIR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKRIR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKRIR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:PRKRIR contains a region (amino acids 86 to 200) of limited homology (24% identity) to the charged domain of HSP90.The THAP domain of the deduced 761-amino acid THAP0 protein includes a C2CH signature, an AVPTIF box, and several other conserved amino acids.
When coexpressed with PKR in yeast, P58(IPK) repressed PKR-mediated EIF2-alpha phosphorylation, inhibiting the normally toxic and growth-suppressive effects associated with PKR function. Conversely, introduction of P52(rIPK) into these strains resulted in restoration of both PKR activity and EIF2-alpha phosphorylation, concomitant with growth suppression due to inhibition of P58(IPK)
