Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PPAT in samples. An antibody specific for PPAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPAT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPAT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:COASY has been shown to interact with P70-S6 Kinase 1. COASY has also been implicated in PI3K signaling, as it was shown to interact with a regulatory subunit of PI3K.
Expressed in all tissues examined including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocyte. Lowest expression in peripheral blood leukocytes and highest in kidney and liver. Isoform 2 is expressed mainly in the brain.Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis.
