Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PPAR-B in samples. An antibody specific for PPAR-B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPAR-B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPAR-B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPAR-B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Peroxisome Proliferator-Activated Receptor gamma is a member of the orphan nuclear receptor family. Oxidized metabolites of linoleic acid, 9-hydroxyctadienoic acid (9-HODE) and 13-HODE are activators and ligands of PPAR-gamma. PPAR-gamma; is expressed in white adipose tissue, intestinal mucosa, colon, spleen, monocytes, macrophages, retina, cartilage, osteoclasts, and skeletal muscle. PPAR-γ plays important roles in lipid and glucose metabolism, and has been implicated in obesity-related metabolic diseases such as hyperlipidemia, insulin resistance, and coronary artery disease. Three known family members are called PPAR alpha, delta, and gamma. Three N-terminal isoforms, called gamma1, gamma 2 and gamma 3, are known to arise by alternative splicing and promoter usage from the PPAR-gamma gene. RXR is an obligate partner for PPAR.
