Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate POR in samples. An antibody specific for POR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:By Southern blot analysis of DNA isolated from a panel of 8 independent human-rodent somatic cell hybrids, Shephard et al. (1989) determined that cytochrome P450 reductase is encoded by a single gene located on 7pter-q22. By in situ hybridization to metaphase chromosomes, they refined the localization to 7q11.2.
In 4 unrelated patients with disordered steroidogenesis including a woman with amenorrhea and 3 children with bony features of Antley-Bixler syndrome, Fluck et al. (2004) found mutations in the POR gene. This was somewhat surprising since the affected individuals lacked apparent disorders of bile acid synthesis or drug metabolism, which also requires P450 enzymes, and knockout of POR is embryonically lethal in mice
