Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate POLN in samples. An antibody specific for POLN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOLN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:POLN is predicted to contain a fingers-thumb-palm structure conserved in the PolA family. Northern blot analysis of human tissues detected an approximately 4-kb transcript in testis, heart, and skeletal muscle, and a 2-kb transcript in heart, liver, skeletal muscle, kidney, and pancreas. The 2-kb transcript was detected in a panel of human cancer cell lines, and the 4-kb transcript was detected only in a chronic myelogenous leukemia cell line. Both POLN and DNA helicase HEL308 showed high expression in human and mouse testis. In situ hybridization of mouse testis tissue localized the highest Poln expression to meiotic spermatocytes and postmeiotic round spermatids, while Hel308 expression occurred in primary spermatocytes but was not detected in round spermatids.
