• Product NameTranscription initiation factor IIB Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Transcription initiation factor IIB polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Transcription initiation factor IIB protein
• Target NameTranscription initiation factor IIB
• Alternative Names    General transcription factor TFIIB S300-II GTF2B TF2B, TFIIB
• Accession No. Swiss-Prot#: Q00403
• UniprotQ00403
• Gene ID2959;
• Calculated MW 35kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.

[1]Cloning of a human gene encoding the general transcription initiation factor IIB.Ha I., Lane W.S., Reinberg D.Nature 352:689-695(1991)[2]Sequence of general transcription factor TFIIB and relationships to other initiation factors.Malik S., Hisatake K

• All lanes:Transcription initiation factor IIB antibody at 2ug/ml Lane 1:HepG2 whole cell lysate Lane 2:Hela whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size:35KDa Observed band size:35KDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting