Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PNRC1 in samples. An antibody specific for PNRC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPNRC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PNRC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PNRC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Proline-rich nuclear receptor coactivator 1 is a protein encoded by the PNRC1 gene.By screening a natural killer (NK) cell cDNA library subtracted with a Jurkat T-cell line, Chen et al. (1995) obtained a cDNA encoding PROL2, which they termed B4-2. The deduced 327-amino acid proline-rich protein has a potential N-terminal SH3-binding domain, a nuclear targeting sequence, and 7 SPxx or TPxx motifs. Northern blot analysis revealed expression of a 1.9-kb transcript in NK, T, and monocyte cell lines, as well as in a variety of other cell lines. Western blot analysis showed expression of a 35-kD protein. The International Radiation Hybrid Mapping Consortium mapped the PROL2 gene to chromosome 6.
