Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PNPLA4 in samples. An antibody specific for PNPLA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPNPLA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PNPLA4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PNPLA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:PNPLA4 encodes a member of the patatin-like family of phospholipases. The encoded enzyme has both triacylglycerol lipase and transacylase activities and may be involved in adipocyte triglyceride homeostasis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome Y.DNA sequencing of a GS2 cDNA clone demonstrated an open reading frame for a basic protein of 253 amino acid residues and an isoelectric point of 9.8. A polymorphic CT dinucleotide repeat was found in the 3-prime untranslated region. The GS2 gene was expressed in all human tissues examined, including heart, brain, placenta, lung, liver, muscle, kidney, pancreas, and spleen. Several GS2 transcripts, ranging in size from 1.1 to 5.8 kb, were found among different tissues, suggesting tissue-specific processing of the GS2 transcript.
