Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate Plin1/Peri/Plin in samples. An antibody specific for Plin1/Peri/Plin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPlin1/Peri/Plin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Plin1/Peri/Plin is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Plin1/Peri/Plin bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Perilipin is a protein that coats lipid droplets in adipocytes, the fat-storing cells in adipose tissue. Perilipin acts as a protective coating from the bodys natural lipases, such as hormone-sensitive lipase,which break triglycerides into glycerol and free fatty acids for use in metabolism, a process called lipolysis.
Perilipin is hyperphosphorylated by PKA following β-adrenergic receptor activation. Phosphorylated perilipin changes conformation, exposing the stored lipids to hormone-sensitive lipase-mediated lipolysis. Although PKA also phosphorylates hormone-sensitive lipase, which can increase PKA activity, the more than 50-fold increase in fat mobilization (triggered by epinephrine) is primarily due to perilipin phosphorylation.
