Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PARS2 in samples. An antibody specific for PARS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPARS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PARS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PARS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:By searching a database for tRNA synthetases, Bonnefond et al. (2005) identified PARS2, which they called MT-PRORS. The deduced 475-amino acid protein has a 47-amino acid mitochondrial targeting signal, resulting in a mature protein of 428 amino acids. PARS2 is a class II amino acid tRNA synthetase, with a C-terminal active-site domain linked to an N-terminal anticodon-binding domain by a short hinge. PARS2 shares no significant similarity with its cytosolic counterpart, EPRS.
Bonnefond et al. (2005) determined that the PARS2 gene contains 1 exon and spans 1.4 kb. By genomic sequence analysis, Bonnefond et al. (2005) mapped the PARS2 gene to chromosome 1.
