Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ODAM in samples. An antibody specific for ODAM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyODAM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ODAM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ODAM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:ODAM is expressed in rodent enamel organ and is likely involved in dental development.Notably, these reagents immunostained normal and malignant ameloblasts and other types of human neoplastic cells, including those of gastric, lung, and breast origin where the presence in the latter was confirmed by in situ hybridization using gene-specific molecular probes. Moreover, significant titers of anti-ODAM IgG antibodies were detected in the sera of patients with these malignancies.ODAM is a developmental antigen that has an essential role in tooth maturation and in the pathogenesis of certain odontogenic and other epithelial neoplasms; ODAM may serve as a novel prognostic biomarker, as well as a potential diagnostic and therapeutic target for patients with breast and other epithelial forms of cancer
