Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate NPAT in samples. An antibody specific for NPAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNPAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NPAT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NPAT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:NPAT lies only 0.5 kb from the 5-prime end of the ATM gene and is transcribed in the opposite direction as ATM.NPAT encodes a 1,427-amino acid protein containing nuclear localization signals and target sites for phosphorylation by cyclin-dependent protein kinases associated with E2F. NPAT has a calculated molecular mass of 154,300 Da. It is relatively serine and threonine rich. The mRNA of NPAT was detected in all human tissues examined and its genomic sequence was strongly conserved through eukaryotes, suggesting that the NPAT gene may be essential for cell maintenance, i.e., a housekeeping gene. The promoter region may be shared by ATM and NPAT and that each gene may influence the expression of the other.
