Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate LAMP3 in samples. An antibody specific for LAMP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAMP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAMP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAMP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:LAMP3 may play a role in dendritic cell function and in adaptive immunity.
By screening a DC subtraction library, de Saint-Vis et al. (1998) isolated a cDNA encoding DCLAMP. Sequence analysis predicted that the 416-amino acid type I integral membrane protein has an N-terminal signal peptide, followed by a 381-amino acid extracellular domain, a hydrophobic transmembrane domain, and a 10-amino acid cytoplasmic domain containing a conserved GY lysosomal targeting motif, characteristic of LAMP family members.
The DCLAMP protein contains multiple N- and O-glycosylation sites. Northern blot and RT-PCR analysis revealed expression of a 3.2-kb transcript in appendix, thymus, lymph node, lung, and weakly in spleen, as well as in dendritic cell lines; expression was weak or undetectable in other cell lines.
