Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate KRT26 in samples. An antibody specific for KRT26 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT26 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT26 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT26 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Keratin is the key structural material making up the outer layer of human skin. It is also the key structural component of hair and nails. Keratin monomers assemble into bundles to form intermediate filaments, which are tough and insoluble and form strong unmineralized tissues found in reptiles, birds, amphibians, and mammals.
KRT26is a member of the keratin superfamily. This keratin protein is a type I keratin that is specific for the inner root sheath of the hair follicle. This gene exists in a cluster with other keratin genes on chromosome 17q21. Strongly expressed in skin and scalp, and weak expression observed in thymus and tongue. In the hair follicle, expression is restricted to the mid- to upper inner root sheath cuticle, being present slightly above the apex of the dermal papilla (at protein level).
