Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate IL4 in samples. An antibody specific for IL4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Interleukin 4 is a pleiotropic cytokine produced primarily by activated T lymphocytes,mast cells and basophils . IL-4 has multiple immune response-modulating functions on a variety of cell types.
It is an important modulator of the differentiation of precursor T helper cells to the Th2 subset that mediates humoral immunity and modulates antibody production. In addition, IL-4 has also been shown to have anti-tumor activity both in vivoand in vitro.The biological effects of IL-4 are mediated by specific cell surface receptor complexes. One type of functional IL-4 receptor complex consists of the IL-4-binding subunit and a second chain, designated the common c chain because it has also been identified as a component of the receptor complexes for IL-4, IL-7, IL-9 and IL-15
