Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate HCN3 in samples. An antibody specific for HCN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHCN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HCN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HCN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:HCN3 conducted both potassium and sodium ions with a 3:1 preference for potassium ions. HCN3 bound cAMP, but unlike other HCNs, its activity was not modulated by intracellular cAMP.The cDNA contains a repetitive element in its 3-prime proximal region, and the deduced protein contains 703 amino acids. RT-PCR ELISA detected high HCN3 expression in adult liver and kidney and fetal liver and brain, intermediate expression in adult whole brain, lung, pancreas, spleen, testis, ovary, and all specific brain regions examined, and little to no expression in heart and skeletal muscle.Expression was high in fetal brain and adult cerebellum and intermediate in nucleus accumbens, thalamus, and pituitary gland, with little to no expression in other brain regions examined.
