Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate HBB in samples. An antibody specific for HBB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHBB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HBB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HBB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Inhibin, beta B is a protein which in humans is encoded by the INHBB gene.INHBB is a subunit of both activin and inhibin, two closely related glycoproteins with opposing biological effects.Inhibins are heterodimeric glycoproteins composed of an α subunit (INHA) and one of two homologous, but distinct, β subunits (βA or βB, this protein).
mRNA for the two subunits has been demonstrated in the testes of adult rats.Inhibin can bind specifically to testicular interstitial cells throughout development and may be an important regulator of Leydig cell testosterone production or interstitial cell function.The inhibin beta B subunit joins the α subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumour-suppressor activity.
