Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate HBA1 in samples. An antibody specific for HBA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHBA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HBA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HBA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Hemoglobin is the iron-containing oxygen-transport metalloprotein in the red blood cells of vertebrates, and the tissues of some invertebrates.In mammals, the protein makes up about 97% of the red blood cells dry content, and around 35% of the total content. Hemoglobin transports oxygen from the lungs or gills to the rest of the body where it releases the oxygen for cell use. It also has a variety of other roles of gas transport and effect-modulation which vary from species to species, and are quite diverse in some invertebrates. Hemoglobin has an oxygen binding capacity of between 1.36 and 1.37 ml O2 per gram of hemoglobin, which increases the total blood oxygen capacity seventyfold.Hemoglobin is found in nonerythroid cells including the A9 dopaminergic neurons in the substantia nigra, macrophages, alveolar cells, and mesangial cells in the kidney.
