• Product Name3-hydroxy-3 methylglutaryl-coenzyme A reductase Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total 3-hydroxy-3 methylglutaryl-coenzyme A reductase polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human 3-hydroxy-3-methylglutaryl-coenzyme A reductase protein
• Target Name3-hydroxy-3 methylglutaryl-coenzyme A reductase
• Alternative Names    HMGCR
• Accession No. Swiss-Prot#: P04035
• UniprotP04035
• Gene ID3156;
• Calculated MW 25kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  Immunohistochemistry: 1:20 - 1:200

Transmembrane glycoprotein that is the rate-limiting enzyme in cholesterol biosynthesis as well as in the biosynthesis of nonsterol isoprenoids that are essential for normal cell function including ubiquinone and geranylgeranyl proteins.

[1] "Human 3-hydroxy-3-methylglutaryl coenzyme A reductase. Conserved domains responsible for catalytic activity and sterol-regulated degradation." Luskey K.L., Stevens B.J. Biol. Chem. 260:10271-10277(1985) [2] "Human HMG-CoA reductase gene."Nakajima

• All lanes:3-hydroxy-3 methylglutaryl-coenzyme A reductase antibody at 2ug/ml Lane 1:Hela whole cell lysate Lane 2:293T whole cell lysate secondary Goat polyclonal to rabbit at 1/10000 dilution predicted band size :25kDa observed band size :25kDa
• Immunohistochemical analysis of paraffin-embedded human placenta using #42206 at dilution of 1:20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting