Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate GRID2IP in samples. An antibody specific for GRID2IP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGRID2IP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GRID2IP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GRID2IP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Glutamate receptor delta-2 (GRID2) is predominantly expressed at parallel fiber-Purkinje cell postsynapses and plays crucial roles in synaptogenesis and synaptic plasticity. GRID2IP1 interacts with GRID2 and may control GRID2 signaling in Purkinje cells.
The L-delphilin isoform contains an N-terminal PDZ domain (PDZ1), followed by a linker region, a second PDZ domain (PDZ2), an FH domain, and a C-terminal coiled-coil domain. Compared with L-delphilin, S-delphilin lacks PDZ1 and the linker region, which are replaced with an N-terminal palmitoylation site. Northern blot analysis of mouse brain showed higher expression of S-delphilin in cerebral cortex than cerebellum, whereas L-delphilin showed higher expression in cerebellum.
