Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate GRIA2 in samples. An antibody specific for GRIA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGRIA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GRIA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GRIA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:GRIA2 belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, GRIA1-4. The subunit encoded by this gene (GRIA2) is subject to RNA editing within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Human and animal studies suggest that pre-mRNA editing is essential for brain function, and defective GRIA2 RNA editing at the Q/R site may be relevant to amyotrophic lateral sclerosis (ALS) etiology. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene, which includes the generation of flip and flop isoforms that vary in their signal transduction properties.
