Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate GPR137B in samples. An antibody specific for GPR137B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGPR137B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPR137B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPR137B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:TM7SF1, a gene that is transcriptionally upregulated during kidney development. RT-PCR detected 3 alternatively spliced TM7SF1 transcripts, which the authors named TM7SF1-long (TM7SF1L), TM7SF1-intermediate (TM7SF1I), and TM7SF1-short (TM7SF1S).
Northern blot analysis detected an approximately 2.4-kb TM7SF1 transcript at highest levels in human kidney and heart, with lower levels detected in brain and placenta. Additional experiments found TM7SF1 expression in spinal cord, caudate nucleus, and putamen. Studies on Wilms tumor samples showed variable TM7SF1 expression, ranging from nearly undetectable levels to an abundant level of expression comparable to that of adult kidney tissue.
