Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate G6PC in samples. An antibody specific for G6PC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyG6PC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for G6PC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of G6PC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Fibroblast growth factor 6 is a protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene displayed oncogenic transforming activity when transfected into mammalian cells.
The mouse homolog of this gene exhibits a restricted expression profile predominantly in the myogenic lineage, which suggested a role in muscle regeneration or differentiation.Marics et al. (1989) showed that the cloned normal FGF6 gene transformed mouse NIH 3T3 fibroblasts using both focus and tumorigenicity assays.
