Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate FUT4 in samples. An antibody specific for FUT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFUT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FUT4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FUT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The sialyl Lewis x oligosaccharide determinant is an essential component of leukocyte counterreceptors for E-selectin and P-selectin mediated adhesions of leukocytes. This oligosaccharide molecule is displayed on the surfaces of granulocytes, monocytes, and natural killer cells. Formation of leukocyte adhesions to these selectins is an early and important step in the process that ultimately allows leukocytes to leave the vascular tree and become recruited into lymphoid tissues and sites of inflammation. FUT7 was expressed in HL-60 cells, a human promyelocytic cell line, and in YT cells, a natural killer-like cell line. Sequence comparisons indicate that the predicted polypeptide sequence shares approximately 38 to 47% overall identity with other FUTs.
