Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate FURIN in samples. An antibody specific for FURIN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFURIN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FURIN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FURIN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Furin is an enzyme which belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a calcium-dependent serine endoprotease that can efficiently cleave precursor proteins at their paired basic amino acid processing sites. Some of its substrates are: proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. A furin-like pro-protein convertase has been implicated in the processing of RGMc (also called hemojuvelin ), a gene involved in a severe iron-overload disorder called juvenile hemochromatosis.
