Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate FGF11 in samples. An antibody specific for FGF11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFGF11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF11 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Fibroblast growth factor 11 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.
The function of this gene has not yet been determined. The expression pattern of the mouse homolog implies a role in nervous system development.A sequence tagged site (STS), which encompassed 1 exon of FHF3, was derived from chromosome 17 and mapped near the BRCA1 gene which is located on 17q21.
