Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate FABP7 in samples. An antibody specific for FABP7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFABP7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FABP7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FABP7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Fatty acid binding protein 7, brain (FABP8; also brain lipid binding protein, BLBP), is a brain fatty acid binding protein. Fatty acid binding proteins (FABPs) are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids?????
