Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ELA3B in samples. An antibody specific for ELA3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyELA3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ELA3B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ELA3B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays..
