Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate DIAPH1 in samples. An antibody specific for DIAPH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDIAPH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DIAPH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DIAPH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:DIAPH1 is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear.This human homolog of diaphanous has approximately 3,800 bp of coding sequence and a 3-prime untranslated region (UTR) of 918 or 1,891 bp.expressed in brain, heart, placenta, lung, kidney, pancreas, liver, and skeletal muscle. A single transcript of 4.7 kb was observed in all tissues, with highest expression in skeletal muscle.
