Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate CHRM3 in samples. An antibody specific for CHRM3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCHRM3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHRM3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRM3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The , also known as the cholinergic receptor, muscarinic 3, is a muscarinic acetylcholine receptor.The M4 muscarinic receptors are located at many places in the body, e.g. smooth muscles, endocrine, in exocrine glands, as well as in the lungs. They are al????
