Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate CHRDL2 in samples. An antibody specific for CHRDL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCHRDL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHRDL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRDL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The deduced 429-amino acid human protein shares 73% homology with the 426-amino acid mouse protein. CHL2 contains an N-terminal signal peptide, followed by 3 chordin (CHRD)-like cysteine-rich repeats (CR1 through CR3) and a C-terminal tail. Northern blot analysis detected high expression of a 2.0-kb CHL2 transcript in adult human uterus, with lower expression in colon, heart, prostate, stomach, and skeletal muscle. Expression was weak in placenta, testis, small intestine, trachea, and bone marrow, and no expression was detected in liver or kidney. In situ hybridization of mouse embryos showed that Chl2 expression was restricted to chondrocytes of developing joint cartilage. In adult mice, Chl2 expression was detected in connective tissues of reproductive organs, with highest expression in uterine myometrium.
