Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate CFHR2 in samples. An antibody specific for CFHR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCFHR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CFHR2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CFHR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:FHR2 encodes a serum protein structurally and immunologically related to complement factor H (CFH). Skerka et al. (1995) isolated and characterized the CFHR2 gene from a YAC library.Skerka et al. (1995) determined that the CFHR2 gene contains 5 exons and spans about 7 kb of genomic DNA.Skerka et al. (1995) demonstrated that the CFHR2 gene and the gene for the beta subunit of coagulation factor XIII (F13B) are located in the same 165-kb YAC DNA. Thus, the 3 structurally related genes F13B, CFHR2, and CFH are linked on chromosome 1 in the RCA (regulators of complement activation) gene cluster.The 5 factor H-related proteins have been identified and shown to be encoded by 5 genes, CFHR1, CFHR2, CFHR3 , CFHR4, and CFHR5, all closely linked to the CFH gene on chromosome 1q31-q32.1
