Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate CBLN1 in samples. An antibody specific for CBLN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCBLN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CBLN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CBLN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Precerebellin, or CBLN1, is the precursor of a conserved brain-specific hexadecapeptide termed cerebellin. CBLN1 and other members of the precerebellin family also contain a C-terminal C1q signature domain that mediates trimeric assembly of atypical collagen complexes. The deduced 193-amino acid protein contains a hydrophobic N terminus followed by the cerebellin sequence. It also contains 3 potential N-glycosylation sites. Over about 145 amino acids in its C terminus, CBLN1 shares 31.3% identity with C1QB , but it lacks N-terminal collagen-like motifs. The cerebellin peptide is flanked by val-arg and glu-pro residues, suggesting that it is not liberated from precerebellin by the classical dibasic amino acid proteolytic mechanism seen in many neuropeptide precursors.
