Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate CaM in samples. An antibody specific for CaM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCaM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CaM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CaM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions.CaM mediates processes such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth and the immune response. CaM is expressed in many cell types and can have different subcellular locations, including the cytoplasm, within organelles, or associated with the plasma or organelle membranes. Many of the proteins that CaM binds are unable to bind calcium themselves, and as such use CaM as a calcium sensor and signal transducer. CaM can also make use of the calcium stores in the endoplasmic reticulum, and the sarcoplasmic reticulum.
