Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate BMP1 in samples. An antibody specific for BMP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBMP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BMP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The BMP1 locus encodes a protein that is capable of inducing formation of cartilage in vivo. Although other bone morphogenetic proteins are members of the TGF-beta superfamily, BMP1 encodes a protein that is not closely related to other known growth factors.BMP1 protein and procollagen C proteinase (PCP), a secreted metalloprotease requiring calcium and needed for cartilage and bone formation, are identical. PCP or BMP1 protein cleaves the C-terminal propeptides of procollagen I, II, and III and its activity is increased by the procollagen C-endopeptidase enhancer protein. The BMP1 gene is expressed as alternatively spliced variants that share an N-terminal protease domain but differ in their C-terminal region.
