Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ASPH in samples. An antibody specific for ASPH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyASPH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ASPH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ASPH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:ASPH is is thought to play an important role in calcium homeostasis. The gene is expressed from two promoters and undergoes extensive alternative splicing. The encoded set of proteins share varying amounts of overlap near their N-termini but have substantial variations in their C-terminal domains resulting in distinct functional properties. The longest isoforms (a and f) include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domain that hydroxylates aspartic acid or asparagine residues in the epidermal growth factor (EGF)-like domains of some proteins, including protein C, coagulation factors VII, IX, and X, and the complement factors C1R and C1S. Other isoforms differ primarily in the C-terminal sequence and lack the hydroxylase domain, and some have been localized to the endoplasmic and sarcoplasmic reticulum.
