Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate AP36 in samples. An antibody specific for AP36 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAP36 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AP36 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AP36 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Apelin gene encodes a pre-proprotein of 77 amino acids,with a signal peptide in the N-terminal region. After translocation into the endoplasmic reticulum and cleavage of the signal peptide, the proprotein of 55 amino acids may generate several active fragments: a 36 amino acid peptide corresponding to the sequence 42-77 (apelin 36), a 17 amino acid peptide corresponding to the sequence 61-77 (apelin 17) and a 13 amino acid peptide corresponding to the sequence 65-77 (apelin 13). This later fragment may also undergo a pyroglutamynation at the level of its N-terminal glutamine residue.Apelin is the endogenous ligand for the G-protein-coupled APJ receptor that is expressed at the surface of some cell types. It is widely expressed in various organs such as the heart, lung, kidney, liver, adipose tissue,gastrointestinal tract, brain, adrenal glands, endothelium, and human plasma.
