Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ANXA2P2 in samples. An antibody specific for ANXA2P2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyANXA2P2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANXA2P2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANXA2P2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Annexin 2 is involved in diverse cellular processes such as cell motility (especially that of the epithelial cells), linkage of membrane-associated protein complexes to the actin cytoskeleton, endocytosis, fibrinolysis, ion channel formation, and cell matrix interactions. It is a calcium-dependent phospholipid-binding protein whose function is to help organize exocytosis of intracellular proteins to the extracellular domain. Annexin II is a pleiotropic protein meaning that its function is dependent on place and time in the body. This protein is a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption.
